anti βactin (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc anti βactin
Anti βactin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 6740 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+%CE%B2actin/beta-Actin+Mouse+mAb/pm41933050-88-51-53
Average 99 stars, based on 6740 article reviews

anti βactin - by Bioz Stars, 2026-10

99/100 stars

Images

Membrane:

Article Title: LncRNA AC040169.1 is regulated by m6A modification and suppresses ferroptosis via SLC7A11 to promote ovarian cancer progression
Article Snippet: Aliquots of protein samples were separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a PVDF membrane (MerckMillipore). .. The membrane was blocked and incubated with anti-METTL14 (E2G9A, CST), anti-SLC7A11 (D2M7A, CST) and anti-βactin (D10A8, CST) overnight, and then incubated with the corresponding secondary antibodies Anti-Mouse IgG, HRP-linked (SA00001-1, Proteintech), Anti-Rabbit IgG, HRP-linked (SA00001-2, Proteintech), β-Actin Mouse mAb (HRP Conjugate) (66009-1-Ig, Proteintech). .. Finally, ImageJ software was selected to analyse the grey scale values of the protein bands.

Article Title: LncRNA AC040169.1 is regulated by m6A modification and suppresses ferroptosis via SLC7A11 to promote ovarian cancer progression.
Article Snippet: Aliquots of protein samples were separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a PVDF membrane (MerckMillipore). .. The membrane was blocked and incubated with anti-METTL14 (E2G9A, CST), antiSLC7A11 (D2M7A, CST) and anti-βactin (D10A8, CST) overnight, and then incubated with the corresponding secondary antibodies Anti-Mouse IgG, HRPlinked (SA00001-1, Proteintech), Anti-Rabbit IgG, HRP-linked (SA00001-2, Proteintech), β-Actin Mouse mAb (HRP Conjugate) ( 66009-1-Ig, Proteintech). .. Finally, ImageJ software was selected to analyse the grey scale values of the protein bands.

Incubation:

Concentration Assay:

Article Title: Lithium attenuates HIV-1 latency reversal in an autophagy-independent way
Article Snippet: .. Antibody concentration: 1:2000 mouse anti-p24 (Bei resources), 1:5000 anti-βactin (CST), 1:5000 mouse anti-GAPDH (CST), 1:1000 anti-p62/SQSTM1 (CST), 1:2000 anti-LC3B (Abcam), 1:2000 rabbit anti-phospho S6K T389 (CST), 1:1000 rabbit anti-S6K Total (CST), 1:2000 mouse anti-GSK-3β Total (CST), 1:2000 rabbit anti-phospho S9 GSK3β (CST), 1:1000 rabbit anti-ATG5 (R&D systems), 1:1000 anti-BECN1 (CST), 1:5000 Goat anti-mouse HRP (Invitrogen), 1:5000 Goat anti-rabbit HRP (Invitrogen), 1:3000 Donkey anti-Rabbit AF647 (Invitrogen), 1:3000 Donkey anti-mouse AF594 (Invitrogen). .. The SMART Vector lentiviral shRNA system (Horizon) was used for gene knockdown against ATG5, Beclin1, or non-target (NT) shRNA sequences.

Western Blot:

Article Title: Detachment-Induced FAK-STAT3-NNMT Inhibits CTCs Anoikis to Promote Breast Cancer Metastasis by Enhancing Fatty Acid Oxidation.
Article Snippet: PPZ(HY0077A) and OA (#HY-N6785) were purchased from MCE. .. The antibodies used for Western blot analysis were as follows: anti- βactin (#4970), anti-P-STAT3(Tyr705) (#9145), anti-STAT3(#9139), anti-FAK(#3285), anti-P-FAK (Tyr397) (#8556), anti-CPT1A (#12252), anti-CD36 (#14347), anti-P-AKT(Ser473) (#4060), anti-AKT(#9272), anti-PARP(#9532), anti-C-myc(#18583), anti-PP2A(#2038), anti-PPAR γ(#2443), anti-ACC(#3676), antiP-ACC(#11818), anti-LXR β(#13519) (Cell Signaling Technology), anti-PPAR α(ab126285), anti-FGF21(ab171941) (Abcam), antimethylation-PP2A(sc-81603) (Santa Cruz Biotechnology), anti-LXR α(14315-1-AP) (Proteintech), and anti-NNMT(1E7) obtained as previously described [ 26 ]. ..

other:

Article Title: Epstein–Barr Virus Silences GSDME and Pyroptosis in Gastric Cancer
Article Snippet: Cells were lysed and mixed with 5× SDS loading buffer (Dynebio, Seoul, Republic of Korea), then heated at 95 ◦C for 5 min.

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